Simultaneous detection of human bocavirus and adenovirus by multiplex real-time PCR in a Belgian paediatric population.
Identifieur interne : 000076 ( Main/Exploration ); précédent : 000075; suivant : 000077Simultaneous detection of human bocavirus and adenovirus by multiplex real-time PCR in a Belgian paediatric population.
Auteurs : N. De Vos [Belgique] ; A. Vankeerberghen ; F. Vaeyens ; K. Van Vaerenbergh ; A. Boel ; H. De BeenhouwerSource :
- European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology [ 1435-4373 ] ; 2009.
Descripteurs français
- KwdFr :
- ADN viral (génétique), ADN viral (isolement et purification), Adénovirus humains (génétique), Adénovirus humains (isolement et purification), Belgique (épidémiologie), Bocavirus humain (génétique), Bocavirus humain (isolement et purification), Comorbidité, Enfant d'âge préscolaire, Facteurs temps, Humains, Infections de l'appareil respiratoire (virologie), Infections à Adenoviridae (diagnostic), Infections à Adenoviridae (épidémiologie), Infections à Parvoviridae (diagnostic), Infections à Parvoviridae (épidémiologie), Metapneumovirus (isolement et purification), Mâle, Nourrisson, Partie nasale du pharynx (virologie), Prévalence, Réaction de polymérisation en chaîne (), Sensibilité et spécificité, Techniques de laboratoire clinique (), Virus de la grippe A (isolement et purification), Virus de la maladie aléoutienne du vison (isolement et purification), Virus respiratoires syncytiaux (isolement et purification).
- MESH :
- diagnostic : Infections à Adenoviridae, Infections à Parvoviridae.
- génétique : ADN viral, Adénovirus humains, Bocavirus humain.
- isolement et purification : ADN viral, Adénovirus humains, Bocavirus humain, Metapneumovirus, Virus de la grippe A, Virus de la maladie aléoutienne du vison, Virus respiratoires syncytiaux.
- virologie : Infections de l'appareil respiratoire, Partie nasale du pharynx.
- épidémiologie : Belgique, Infections à Adenoviridae, Infections à Parvoviridae.
- Comorbidité, Enfant d'âge préscolaire, Facteurs temps, Humains, Mâle, Nourrisson, Prévalence, Réaction de polymérisation en chaîne, Sensibilité et spécificité, Techniques de laboratoire clinique.
- Wicri :
- geographic : Belgique.
English descriptors
- KwdEn :
- Adenoviridae Infections (diagnosis), Adenoviridae Infections (epidemiology), Adenoviruses, Human (genetics), Adenoviruses, Human (isolation & purification), Aleutian Mink Disease Virus (isolation & purification), Belgium (epidemiology), Child, Preschool, Clinical Laboratory Techniques (methods), Comorbidity, DNA, Viral (genetics), DNA, Viral (isolation & purification), Human bocavirus (genetics), Human bocavirus (isolation & purification), Humans, Infant, Influenza A virus (isolation & purification), Male, Metapneumovirus (isolation & purification), Nasopharynx (virology), Parvoviridae Infections (diagnosis), Parvoviridae Infections (epidemiology), Polymerase Chain Reaction (methods), Prevalence, Respiratory Syncytial Viruses (isolation & purification), Respiratory Tract Infections (virology), Sensitivity and Specificity, Time Factors.
- MESH :
- chemical , genetics : DNA, Viral.
- geographic , epidemiology : Belgium.
- diagnosis : Adenoviridae Infections, Parvoviridae Infections.
- epidemiology : Adenoviridae Infections, Parvoviridae Infections.
- genetics : Adenoviruses, Human, Human bocavirus.
- isolation & purification : Adenoviruses, Human, Aleutian Mink Disease Virus, DNA, Viral, Human bocavirus, Influenza A virus, Metapneumovirus, Respiratory Syncytial Viruses.
- methods : Clinical Laboratory Techniques, Polymerase Chain Reaction.
- virology : Nasopharynx, Respiratory Tract Infections.
- Child, Preschool, Comorbidity, Humans, Infant, Male, Prevalence, Sensitivity and Specificity, Time Factors.
Abstract
Since the discovery of human bocavirus (hBoV), the virus has been detected worldwide in respiratory tract samples from young children by various polymerase chain reaction (PCR) assays and real-time PCRs (Q-PCR). Until now, no data have been reported on the presence of hBoV in Belgium and the detection of hBoV in a multiplex Q-PCR setting has not been described. The aim of this study was to develop a fast and reliable multiplex Q-PCR for the simultaneous detection of hBoV DNA and adenovirus (AdV) DNA. During the winter of 2004-2005, 445 nasopharyngeal aspirates (NPAs) were analysed from 404 Belgian children up to 5 years old with acute respiratory tract infections (ARTIs). (Co)infections with hBoV, AdV, respiratory syncytial virus (RSV), human metapneumovirus (hMPV) and influenza A virus were investigated. A viral agent was detected in 61% (n = 272/445) of the NPAs. Multiplex Q-PCR found a prevalence of 11% (n = 51/445) hBoV and 13% (n = 58/445) AdV. Coinfections were more frequently found with AdV (62%; n = 36/58) than with hBoV (49%; n = 25/51). Follow-up samples were available from 22 patients with ARTIs. In three patients, hBoV DNA persisted for one month. Multiplex Q-PCR may help in closing the diagnostic gap by addressing a broader range of potential respiratory pathogens.
DOI: 10.1007/s10096-009-0780-y
PubMed: 19705175
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Simultaneous detection of human bocavirus and adenovirus by multiplex real-time PCR in a Belgian paediatric population.</title><author><name sortKey="De Vos, N" sort="De Vos, N" uniqKey="De Vos N" first="N" last="De Vos">N. De Vos</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Clinical Biology, Department of Microbiology and Molecular Biology, Onze Lieve Vrouw (OLV) Hospital, Moorselbaan 164, 9300, Aalst, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Laboratory of Clinical Biology, Department of Microbiology and Molecular Biology, Onze Lieve Vrouw (OLV) Hospital, Moorselbaan 164, 9300, Aalst</wicri:regionArea><wicri:noRegion>Aalst</wicri:noRegion></affiliation></author><author><name sortKey="Vankeerberghen, A" sort="Vankeerberghen, A" uniqKey="Vankeerberghen A" first="A" last="Vankeerberghen">A. Vankeerberghen</name></author><author><name sortKey="Vaeyens, F" sort="Vaeyens, F" uniqKey="Vaeyens F" first="F" last="Vaeyens">F. Vaeyens</name></author><author><name sortKey="Van Vaerenbergh, K" sort="Van Vaerenbergh, K" uniqKey="Van Vaerenbergh K" first="K" last="Van Vaerenbergh">K. Van Vaerenbergh</name></author><author><name sortKey="Boel, A" sort="Boel, A" uniqKey="Boel A" first="A" last="Boel">A. Boel</name></author><author><name sortKey="De Beenhouwer, H" sort="De Beenhouwer, H" uniqKey="De Beenhouwer H" first="H" last="De Beenhouwer">H. De Beenhouwer</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19705175</idno><idno type="pmid">19705175</idno><idno type="doi">10.1007/s10096-009-0780-y</idno><idno type="wicri:Area/Main/Corpus">000081</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000081</idno><idno type="wicri:Area/Main/Curation">000081</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000081</idno><idno type="wicri:Area/Main/Exploration">000081</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Simultaneous detection of human bocavirus and adenovirus by multiplex real-time PCR in a Belgian paediatric population.</title><author><name sortKey="De Vos, N" sort="De Vos, N" uniqKey="De Vos N" first="N" last="De Vos">N. De Vos</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Clinical Biology, Department of Microbiology and Molecular Biology, Onze Lieve Vrouw (OLV) Hospital, Moorselbaan 164, 9300, Aalst, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Laboratory of Clinical Biology, Department of Microbiology and Molecular Biology, Onze Lieve Vrouw (OLV) Hospital, Moorselbaan 164, 9300, Aalst</wicri:regionArea><wicri:noRegion>Aalst</wicri:noRegion></affiliation></author><author><name sortKey="Vankeerberghen, A" sort="Vankeerberghen, A" uniqKey="Vankeerberghen A" first="A" last="Vankeerberghen">A. Vankeerberghen</name></author><author><name sortKey="Vaeyens, F" sort="Vaeyens, F" uniqKey="Vaeyens F" first="F" last="Vaeyens">F. Vaeyens</name></author><author><name sortKey="Van Vaerenbergh, K" sort="Van Vaerenbergh, K" uniqKey="Van Vaerenbergh K" first="K" last="Van Vaerenbergh">K. Van Vaerenbergh</name></author><author><name sortKey="Boel, A" sort="Boel, A" uniqKey="Boel A" first="A" last="Boel">A. Boel</name></author><author><name sortKey="De Beenhouwer, H" sort="De Beenhouwer, H" uniqKey="De Beenhouwer H" first="H" last="De Beenhouwer">H. De Beenhouwer</name></author></analytic><series><title level="j">European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology</title><idno type="eISSN">1435-4373</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adenoviridae Infections (diagnosis)</term><term>Adenoviridae Infections (epidemiology)</term><term>Adenoviruses, Human (genetics)</term><term>Adenoviruses, Human (isolation & purification)</term><term>Aleutian Mink Disease Virus (isolation & purification)</term><term>Belgium (epidemiology)</term><term>Child, Preschool</term><term>Clinical Laboratory Techniques (methods)</term><term>Comorbidity</term><term>DNA, Viral (genetics)</term><term>DNA, Viral (isolation & purification)</term><term>Human bocavirus (genetics)</term><term>Human bocavirus (isolation & purification)</term><term>Humans</term><term>Infant</term><term>Influenza A virus (isolation & purification)</term><term>Male</term><term>Metapneumovirus (isolation & purification)</term><term>Nasopharynx (virology)</term><term>Parvoviridae Infections (diagnosis)</term><term>Parvoviridae Infections (epidemiology)</term><term>Polymerase Chain Reaction (methods)</term><term>Prevalence</term><term>Respiratory Syncytial Viruses (isolation & purification)</term><term>Respiratory Tract Infections (virology)</term><term>Sensitivity and Specificity</term><term>Time Factors</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN viral (génétique)</term><term>ADN viral (isolement et purification)</term><term>Adénovirus humains (génétique)</term><term>Adénovirus humains (isolement et purification)</term><term>Belgique (épidémiologie)</term><term>Bocavirus humain (génétique)</term><term>Bocavirus humain (isolement et purification)</term><term>Comorbidité</term><term>Enfant d'âge préscolaire</term><term>Facteurs temps</term><term>Humains</term><term>Infections de l'appareil respiratoire (virologie)</term><term>Infections à Adenoviridae (diagnostic)</term><term>Infections à Adenoviridae (épidémiologie)</term><term>Infections à Parvoviridae (diagnostic)</term><term>Infections à Parvoviridae (épidémiologie)</term><term>Metapneumovirus (isolement et purification)</term><term>Mâle</term><term>Nourrisson</term><term>Partie nasale du pharynx (virologie)</term><term>Prévalence</term><term>Réaction de polymérisation en chaîne ()</term><term>Sensibilité et spécificité</term><term>Techniques de laboratoire clinique ()</term><term>Virus de la grippe A (isolement et purification)</term><term>Virus de la maladie aléoutienne du vison (isolement et purification)</term><term>Virus respiratoires syncytiaux (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Viral</term></keywords><keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>Belgium</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Adenoviridae Infections</term><term>Parvoviridae Infections</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections à Adenoviridae</term><term>Infections à Parvoviridae</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Adenoviridae Infections</term><term>Parvoviridae Infections</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Adenoviruses, Human</term><term>Human bocavirus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN viral</term><term>Adénovirus humains</term><term>Bocavirus humain</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Adenoviruses, Human</term><term>Aleutian Mink Disease Virus</term><term>DNA, Viral</term><term>Human bocavirus</term><term>Influenza A virus</term><term>Metapneumovirus</term><term>Respiratory Syncytial Viruses</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ADN viral</term><term>Adénovirus humains</term><term>Bocavirus humain</term><term>Metapneumovirus</term><term>Virus de la grippe A</term><term>Virus de la maladie aléoutienne du vison</term><term>Virus respiratoires syncytiaux</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Clinical Laboratory Techniques</term><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections de l'appareil respiratoire</term><term>Partie nasale du pharynx</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Nasopharynx</term><term>Respiratory Tract Infections</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Belgique</term><term>Infections à Adenoviridae</term><term>Infections à Parvoviridae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Child, Preschool</term><term>Comorbidity</term><term>Humans</term><term>Infant</term><term>Male</term><term>Prevalence</term><term>Sensitivity and Specificity</term><term>Time Factors</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Comorbidité</term><term>Enfant d'âge préscolaire</term><term>Facteurs temps</term><term>Humains</term><term>Mâle</term><term>Nourrisson</term><term>Prévalence</term><term>Réaction de polymérisation en chaîne</term><term>Sensibilité et spécificité</term><term>Techniques de laboratoire clinique</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Belgique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Since the discovery of human bocavirus (hBoV), the virus has been detected worldwide in respiratory tract samples from young children by various polymerase chain reaction (PCR) assays and real-time PCRs (Q-PCR). Until now, no data have been reported on the presence of hBoV in Belgium and the detection of hBoV in a multiplex Q-PCR setting has not been described. The aim of this study was to develop a fast and reliable multiplex Q-PCR for the simultaneous detection of hBoV DNA and adenovirus (AdV) DNA. During the winter of 2004-2005, 445 nasopharyngeal aspirates (NPAs) were analysed from 404 Belgian children up to 5 years old with acute respiratory tract infections (ARTIs). (Co)infections with hBoV, AdV, respiratory syncytial virus (RSV), human metapneumovirus (hMPV) and influenza A virus were investigated. A viral agent was detected in 61% (n = 272/445) of the NPAs. Multiplex Q-PCR found a prevalence of 11% (n = 51/445) hBoV and 13% (n = 58/445) AdV. Coinfections were more frequently found with AdV (62%; n = 36/58) than with hBoV (49%; n = 25/51). Follow-up samples were available from 22 patients with ARTIs. In three patients, hBoV DNA persisted for one month. Multiplex Q-PCR may help in closing the diagnostic gap by addressing a broader range of potential respiratory pathogens.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19705175</PMID><DateCompleted><Year>2010</Year><Month>01</Month><Day>07</Day></DateCompleted><DateRevised><Year>2020</Year><Month>03</Month><Day>24</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1435-4373</ISSN><JournalIssue CitedMedium="Internet"><Volume>28</Volume><Issue>11</Issue><PubDate><Year>2009</Year><Month>Nov</Month></PubDate></JournalIssue><Title>European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology</Title><ISOAbbreviation>Eur. J. Clin. Microbiol. Infect. Dis.</ISOAbbreviation></Journal><ArticleTitle>Simultaneous detection of human bocavirus and adenovirus by multiplex real-time PCR in a Belgian paediatric population.</ArticleTitle><Pagination><MedlinePgn>1305-10</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s10096-009-0780-y</ELocationID><Abstract><AbstractText>Since the discovery of human bocavirus (hBoV), the virus has been detected worldwide in respiratory tract samples from young children by various polymerase chain reaction (PCR) assays and real-time PCRs (Q-PCR). Until now, no data have been reported on the presence of hBoV in Belgium and the detection of hBoV in a multiplex Q-PCR setting has not been described. The aim of this study was to develop a fast and reliable multiplex Q-PCR for the simultaneous detection of hBoV DNA and adenovirus (AdV) DNA. During the winter of 2004-2005, 445 nasopharyngeal aspirates (NPAs) were analysed from 404 Belgian children up to 5 years old with acute respiratory tract infections (ARTIs). (Co)infections with hBoV, AdV, respiratory syncytial virus (RSV), human metapneumovirus (hMPV) and influenza A virus were investigated. A viral agent was detected in 61% (n = 272/445) of the NPAs. Multiplex Q-PCR found a prevalence of 11% (n = 51/445) hBoV and 13% (n = 58/445) AdV. Coinfections were more frequently found with AdV (62%; n = 36/58) than with hBoV (49%; n = 25/51). Follow-up samples were available from 22 patients with ARTIs. In three patients, hBoV DNA persisted for one month. Multiplex Q-PCR may help in closing the diagnostic gap by addressing a broader range of potential respiratory pathogens.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>De Vos</LastName><ForeName>N</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Laboratory of Clinical Biology, Department of Microbiology and Molecular Biology, Onze Lieve Vrouw (OLV) Hospital, Moorselbaan 164, 9300, Aalst, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vankeerberghen</LastName><ForeName>A</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Vaeyens</LastName><ForeName>F</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Van Vaerenbergh</LastName><ForeName>K</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Boel</LastName><ForeName>A</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>De Beenhouwer</LastName><ForeName>H</ForeName><Initials>H</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D023362">Evaluation Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2009</Year><Month>08</Month><Day>25</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Eur J Clin Microbiol Infect Dis</MedlineTA><NlmUniqueID>8804297</NlmUniqueID><ISSNLinking>0934-9723</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004279">DNA, Viral</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000257" MajorTopicYN="N">Adenoviridae Infections</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName><QualifierName UI="Q000453" MajorTopicYN="Y">epidemiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000260" MajorTopicYN="N">Adenoviruses, Human</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000454" MajorTopicYN="N">Aleutian Mink Disease Virus</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001530" MajorTopicYN="N" Type="Geographic">Belgium</DescriptorName><QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002675" MajorTopicYN="N">Child, Preschool</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019411" MajorTopicYN="N">Clinical Laboratory Techniques</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015897" MajorTopicYN="N">Comorbidity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004279" MajorTopicYN="N">DNA, Viral</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D056661" MajorTopicYN="N">Human bocavirus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007223" MajorTopicYN="N">Infant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009980" MajorTopicYN="N">Influenza A virus</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D029121" MajorTopicYN="N">Metapneumovirus</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009305" MajorTopicYN="N">Nasopharynx</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010322" MajorTopicYN="N">Parvoviridae Infections</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName><QualifierName UI="Q000453" MajorTopicYN="Y">epidemiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015995" MajorTopicYN="N">Prevalence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012136" MajorTopicYN="N">Respiratory Syncytial Viruses</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012141" MajorTopicYN="N">Respiratory Tract Infections</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2009</Year><Month>03</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2009</Year><Month>07</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>8</Month><Day>26</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>8</Month><Day>26</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>1</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19705175</ArticleId><ArticleId IdType="doi">10.1007/s10096-009-0780-y</ArticleId><ArticleId IdType="pmc">PMC7087762</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Clin Infect Dis. 2006 Sep 1;43(5):585-92</Citation><ArticleIdList><ArticleId IdType="pubmed">16886150</ArticleId></ArticleIdList></Reference><Reference><Citation>Clin Microbiol Infect. 2007 May;13(5):504-9</Citation><ArticleIdList><ArticleId IdType="pubmed">17263836</ArticleId></ArticleIdList></Reference><Reference><Citation>Clin Infect Dis. 2007 Apr 1;44(7):904-10</Citation><ArticleIdList><ArticleId IdType="pubmed">17342639</ArticleId></ArticleIdList></Reference><Reference><Citation>Emerg Infect Dis. 2007 Apr;13(4):636-7</Citation><ArticleIdList><ArticleId IdType="pubmed">17553287</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Microbiol. 2006 Sep;44(9):3231-5</Citation><ArticleIdList><ArticleId IdType="pubmed">16954253</ArticleId></ArticleIdList></Reference><Reference><Citation>Clin Microbiol Rev. 2008 Apr;21(2):291-304, table of contents</Citation><ArticleIdList><ArticleId IdType="pubmed">18400798</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Virol. 2007 Nov;40 Suppl 1:S24-30</Citation><ArticleIdList><ArticleId IdType="pubmed">18162251</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2005 Sep 6;102(36):12891-6</Citation><ArticleIdList><ArticleId IdType="pubmed">16118271</ArticleId></ArticleIdList></Reference><Reference><Citation>J Infect Dis. 2006 Nov 1;194(9):1276-82</Citation><ArticleIdList><ArticleId IdType="pubmed">17041854</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Virol. 2007 Nov;40(3):229-35</Citation><ArticleIdList><ArticleId IdType="pubmed">17851126</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Virol. 2006 Jan;35(1):99-102</Citation><ArticleIdList><ArticleId IdType="pubmed">16257260</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Microbiol. 2007 Jul;45(7):2116-22</Citation><ArticleIdList><ArticleId IdType="pubmed">17475762</ArticleId></ArticleIdList></Reference><Reference><Citation>Clin Microbiol Rev. 1996 Jan;9(1):18-33</Citation><ArticleIdList><ArticleId IdType="pubmed">8665474</ArticleId></ArticleIdList></Reference><Reference><Citation>Acta Clin Belg. 2008 Sep-Oct;63(5):329-34</Citation><ArticleIdList><ArticleId IdType="pubmed">19186566</ArticleId></ArticleIdList></Reference><Reference><Citation>Pediatrics. 2008 Mar;121(3):e631-7</Citation><ArticleIdList><ArticleId IdType="pubmed">18310182</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Virol. 2008 Jul;42(3):233-43</Citation><ArticleIdList><ArticleId IdType="pubmed">18406664</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Virol. 2008 Jan;41(1):29-33</Citation><ArticleIdList><ArticleId IdType="pubmed">18055252</ArticleId></ArticleIdList></Reference><Reference><Citation>J Infect Dis. 2007 Apr 1;195(7):1038-45</Citation><ArticleIdList><ArticleId IdType="pubmed">17330795</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Virol. 2007 Sep;40(1):9-14</Citation><ArticleIdList><ArticleId IdType="pubmed">17714984</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Pediatr. 2008 Feb;20(1):62-6</Citation><ArticleIdList><ArticleId IdType="pubmed">18197041</ArticleId></ArticleIdList></Reference><Reference><Citation>J Med Virol. 2003 Jun;70(2):228-39</Citation><ArticleIdList><ArticleId IdType="pubmed">12696109</ArticleId></ArticleIdList></Reference><Reference><Citation>Eur J Clin Microbiol Infect Dis. 2007 Feb;26(2):147-9</Citation><ArticleIdList><ArticleId IdType="pubmed">17216422</ArticleId></ArticleIdList></Reference><Reference><Citation>Clin Infect Dis. 2006 Aug 1;43(3):283-8</Citation><ArticleIdList><ArticleId IdType="pubmed">16804840</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Microbiol. 2006 Mar;44(3):1132-4</Citation><ArticleIdList><ArticleId IdType="pubmed">16517912</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Infect Dis. 2006 Jul 11;6:109</Citation><ArticleIdList><ArticleId IdType="pubmed">16834781</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Belgique</li></country></list><tree><noCountry><name sortKey="Boel, A" sort="Boel, A" uniqKey="Boel A" first="A" last="Boel">A. Boel</name><name sortKey="De Beenhouwer, H" sort="De Beenhouwer, H" uniqKey="De Beenhouwer H" first="H" last="De Beenhouwer">H. De Beenhouwer</name><name sortKey="Vaeyens, F" sort="Vaeyens, F" uniqKey="Vaeyens F" first="F" last="Vaeyens">F. Vaeyens</name><name sortKey="Van Vaerenbergh, K" sort="Van Vaerenbergh, K" uniqKey="Van Vaerenbergh K" first="K" last="Van Vaerenbergh">K. Van Vaerenbergh</name><name sortKey="Vankeerberghen, A" sort="Vankeerberghen, A" uniqKey="Vankeerberghen A" first="A" last="Vankeerberghen">A. Vankeerberghen</name></noCountry><country name="Belgique"><noRegion><name sortKey="De Vos, N" sort="De Vos, N" uniqKey="De Vos N" first="N" last="De Vos">N. De Vos</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/GrippeBelgiqueV4/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000076 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000076 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= GrippeBelgiqueV4
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:19705175
|texte= Simultaneous detection of human bocavirus and adenovirus by multiplex real-time PCR in a Belgian paediatric population.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:19705175" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a GrippeBelgiqueV4
| This area was generated with Dilib version V0.6.35. |  |